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Questions related from Leah Maharaj
I have been using DNASP to run analysis on my sequences, but with linkage disequilibrium the analysis does not run and I get the message in error(2).
22 July 2022 8,467 0 View
I have agarose gel slices with PCR products in them for purification and sequencing. I need to send the slices to another lab for processing which may take up to 5 working days. Is it okay to...
04 July 2022 727 4 View
I am using nested PCR to determine the alleles present in my parasite population for a specific gene. This has gone well, but one gene has given me an issue. The initial PCR for gene MSP1...
28 June 2022 8,885 3 View
I have sequenced purified amplicons by Sanger sequencing. I want to create a consensus sequence contig for each amplicon. Using the .ab1 files I trimmed the ends of the forward and reverse...
17 April 2022 6,517 6 View
I have outsourced Sanger sequencing of my purified PCR products. Looking at the chromatogram, I know to trim the ends. However, the ends that need to be trimmed according to the quality scores are...
08 April 2022 7,552 3 View
I have several blood samples in EDTA tubes. However, I now require serum from these samples for ELISA. The protocol I know of involves allowing the blood to clot, and then spinning it down in a...
17 February 2022 178 4 View
I am working with malaria-infected blood samples. Currently I am using PCR to determine allele frequencies of my genes of interest. I would like to determine the specific antibody responses (IgG)...
09 November 2021 1,444 2 View
I have a sample mixed with human and parasite DNA that is being sequenced (whole genome sequencing) When the reads come back, I am expecting a combination of human and parasite DNA. Is the best...
14 October 2021 6,035 3 View
I've been trying to run PCR on blood infected with Plasmodium which has low levels of the parasite. The PCR target is a gene of Plasmodium. Has anyone had experience with this low parasitaemia...
02 July 2021 2,286 2 View
I am using the above kit to extract DNA from whole blood. The protocol requires PBS (pH 7.2 50mM potassium phosphate and 150mM NaCl). I am struggling to obtain potassium phosphate. Has anyone...
26 April 2021 6,922 0 View
I am looking to measure allele-specific immune responses for specific antigens. After identifying the most prominent alleles in a population, I am looking to express them in mice and then measure...
04 September 2020 9,423 1 View
