How to detect lipofuscin autofluorescence in paraffin embedded tissues?

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Is Anti-Albumin antibody (ab106582) a good antibody for Immunofluorescence/IHC?

I recently tested Abcam's anti-albumin antibody (ab106582) on paraffin-embedded sections of mice brain. Unfortunately, the staining is inconsistent and unreliable. Does anyone use this or another...

03 May 2020 5,122 5 View

What is the best antibody for senescence, for IHC on paraffin-embedded tissue?

I am currently working with mice brain sections paraffin-embedded. I tried several antibodies with different protocols, for p21, H2AX and p16. I never had good staining in these sections. Does...

19 February 2020 3,862 4 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

Ellen A G Chernoff

From one of my papers: “Autofluorescence of lipofuscin has an emission spectrum in the region of 430–490 nm, with a maximum in the yellow range (92). This would bleed through typical green fluorescence filters.”. Ref. 92 is

H. Assay of fluorescent lipid peroxidation products. Methods Enzymol. (1994) 233:338–46. doi: 10.1016/S0076-6879(94)33039-5. (in Discussion of my paper, https://www.frontiersin.org/articles/10.3389/fimmu.2019.02558/full)

Carolina Caetano

Thank you Ellen A G Chernoff !