I'm working on validation of the microarray date by using qPCR. There are some types of lncRNAs , including lincRNA, nature antisense, intronic antisense, exon lncRNA. But there is a problem, my primers are not specific. Can anyone tell me some principle of designing primers?
Hello, were you able to design primers for lincRNA? I am struggling on the same
Hey Li, did you figure out how to design lncRNA primers? If yes, I may need your help.
I recently validated some of my intergenic and intronic/exonic lncRNAs. The one problem I faced was their short segment matching to some of the protein coding genes. I did alignment and tried to avoid that matching region from amplicon. Sometimes its hard to see amplification due to template concentration. I suggest using undiluted cDNA template.
Dear All,
Could you get a good amplification in this case? We have a similar set of lncRNAs but we do have a gap instead of matching regions. We are wondering what would be the amplification.
Looking forward
Prash
Hello,
Where you able to figure out the answer? I am struggling with the same issue.
I would really appreciate if you let me know. Thanks in advance.
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