We want to design primers for CpG islands (promoter) of desired genes.
There are some issues that I should mention:
1- We want to analyze the ctDNA
2- We planned to carry out the essay by BSP-sequencing (Not whole genome)
What can we do now?
Joshua Beytebiere
If you are looking to do bisulfite PCR you will want to design primers against the original sequence and the completely converted sequence. Here is a paper that may help get you started: Article Methprimer: Designing Primers for Methylation PCRs.
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Arash Moradi
Joshua Beytebiere Dear Dr. Beytebiere thank you for your consideration
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