Fixed UMI compared with random UMI is becoming more commonly used in library preparation, just like IDT Prism kit and KAPA Universal UMI Adapter.
I just want to konw the designing principle. Can someone friendly answer this question?
Hi all, I need help. I have been trying to assess p-syk (65E4) in THP1 cell extracts unsuccessfully via western-blotting. Anyone has any tricks I should try? So far I have been using PVDF...
11 September 2023 1,300 1 View
Can anyone tell me about the exact concentration of Agilent SureSelect NGS Panel(RNA probe)?
06 August 2023 618 2 View
Hello, I am looking for help on running my SDS-PAGE gel. I am using a Bis-Tris 4-12% gel with 45uL sample and am having trouble getting the best separation between my bands What are your...
17 October 2022 5,555 0 View
Hi, all. I am using G*power to perform a sensitivity analysis for a one-way MANOVA. The analysis suggested my study had a minimum detectable effect size of f^2(V) = .01. I was not quite sure about...
15 October 2022 1,456 4 View
Hi all, I have just used mouse brain samples to investigate levels of GAD65 via western blotting (ab239372). I got very specific staining, but the band appeared to be in a different weight around...
05 October 2021 7,066 3 View
We have CX3CR1-GFP expressing mice and want to collect intestine tissue for IHC analysis. I am not sure how to process the tissue as too much time in PFA may quench the fluorescence. Anyone out...
12 May 2021 3,690 3 View
Investigating the difference in business strategies between ethical marketplaces and traditional online marketplaces such as amazon
27 July 2020 7,509 3 View
Seeking specialists in this mutation. Historical data on patients’ cognitive development and mortality rates.
27 June 2020 4,107 0 View
Hi everyone, I have a technical question. I am performing some TEER measurements of ex vivo intestine tissues on ussing chambers. The problem is as I measured current I kept the lumen side with...
15 January 2020 3,326 0 View
Hello, I am looking for a nuclear stain to use on Arabidopsis whole roots, but it has to be non-toxic and not effect development. I was thinking about Hoechst33342 - lipophillic, and stains...
10 December 2019 4,421 0 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
For a grid connected BESS (lets say 100 MW/200 MWh system, LFP battery), if we charge the BESS in such a way that whenever we can access power from the solar that will charge the BESS. Hence it...
01 August 2024 9,903 7 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
HI there, I've came across several articles discuss about random audit an Non random to tax evasion or compliance. Most of the articles is relating about effect of audit (random or non random)...
31 July 2024 5,309 7 View
I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...
31 July 2024 2,150 0 View
When I run autogrid4 it says: autogrid4: ERROR: Unknown receptor type: "Cr" -- Add parameters for it to the parameter library first! Look forward to your reply.Thank you so much!
29 July 2024 488 0 View
Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...
28 July 2024 6,938 1 View
Gene sequencing related trouble shooting
25 July 2024 4,149 2 View