How to design circular RNA primers?can we add extra bases at the 5' end in order for adjusting there Tm

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Alfonso Saera-Vila

what do you mean with circular primers? could you explain your experiment a little bit more?

Parul Sahu

Alfonso Saera-Vila I am seeking for strategy for designing divergent primers using for circularRNA

Serkan Önder

Dear Parul, you do not need to do extra things for circular RNA. You can already use normal primer settings for this applications.

Serkan Önder ,Thank you for adding your answer.but how we will manage the Tm of sequence

You can use the primer 3 software for design your own primer pairs and you can manage the Tm degrees easyly. As you know it is free.