24 February 2018 3 6K Report

What should be the simplest model for a wireless channel when the traffic intensity is low and surrounding scatter is also low? Is considering Nakagami-m channel be a suitable choice?

More Samarendra Nath Sur's questions See All
Non-parametric tests: PermANOVAs, or Kruskal-Wallis test?

Hello, Because my data has non-normal distributions, I understand I need to run non-parametric tests. My data includes multiple samples from several distant collection sites. Under what...

22 January 2019 5,467 5 View

What evalues are recommended for BLASTing fungal sequences?

Hello, I'm using the command line to NCBI BLAST fungal DNA sequences (Table-C1, https://www.ncbi.nlm.nih.gov/books/NBK279684/). The default evalue is 10.0, but one paper I'm following used...

17 November 2018 1,031 3 View

Grouping read IDs by sample names, and sorting samples into separate fasta files?

Dear colleagues, I pooled together dozens of samples into one library, and sequenced them together. This yielded millions of reads, with the sequencer assigning each unique read-IDs. While the...

08 October 2018 5,513 4 View

What are acceptable thresholds when using BLASTn on fungal DNA sequences?

Hello, I'd like to know your opinions on identifying fungal DNA sequences using the BLASTn with regards to Max Score, Total Score, Query Cover, E-value, and Identity match %? What are common...

06 July 2018 9,896 1 View

Similarity thresholds for fungal OTUs?

Hello, I understand there's disagreement regarding the similarity threshold for fungal OTUs, be it 95%, 97%, or 90-99%. In the literature, anyone of these threshold may be cast as adequate or too...

01 June 2018 3,308 4 View

Ligations: Using DNA ligases and buffers of E. coli, and/or T4?

Hello, I'm following Thermofisher's "Ion 16S Metagenomics Kit User Guide", and I want to ligate Ion P1 Adaptors, and an Ion Xpress Barcode onto dsDNA amplicons for ion torrent sequencing (p. 16,...

29 March 2018 2,018 2 View

Removal of excessive primer dimers: Use multiple sessions of Ampure XP bead purification?

Hello all, Previously after completing my PCRs, their electrophoresis gels indicated all PCRs had primer dimers. I am having difficulty ridding my PCRs of dimers as well as anything 100...

14 December 2017 2,943 4 View

Can I avoid diluting my PCRs to equal molar concentrations before I pool them?

Background: First, I want to sequence fungal DNA collected from samples of over 100 individual trees. Each PCR sample was amplified with an unique barcoded combination of forward and reverse...

12 December 2017 5,083 2 View

Handling PCR inhibitors: Combinations of BSA, Tween-20, MgCl2, DMSO, and/or Betaine? Also GC content of fungal sequences?

Hello all, I am trying to optimize my PCRs to amplify fungal DNA isolated from extracts of mostly plant DNA. I'm using ITS-1F and ITS-2R for my forward and reverse primers, respectively. Past...

29 October 2017 5,299 6 View

How to determine the Tm of barcoded primers?

Hi all, I need help calculating the melting temperature (Tm) of barcoded primers for PCRs. I am using two barcoded primers to separate dozens of samples' DNA sequences. Using the equation...

06 September 2017 2,650 3 View

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After a reference from Int J Body Compos Res, can any body help?

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How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

What is involved in morphological changes of SEM images of a substance?

In the sem analysis of CaCO3 nanaoparticles, the morphology images showed spherical shaped particles but the proposed morphology for these type of nanoparticles is trigonal. I prepared the...

01 March 2021 1,960 4 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Joint behaviour in Abaqus is not captured well?

I am working on roof joint and trying to simulate the behaviour in Abaqus. I am successful in running the model but the final graph does not follow the experimental curve in the negative direction...

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How can I make stacked bar graph with scatter plot?

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01 March 2021 9,383 1 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

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