I want my gene-sequenced data to dock so I can design a new in-silico drug. I really need help. If someone can do that for me or help me with then let me know.
I'm a PhD Student. From Northwest Agriculture and Forestry University China. My Problem is this I start my work on bhlh79 transcription factor gene. I do my Y2H 5 time but the colonies appear on...
30 July 2024 1,412 8 View
Dear friends! I am working on finding motifs by using MEME Suite in COX1 gene. Kindly help me. How to proceed further? What measures should I see for?
05 May 2024 2,740 2 View
I am trying to calculate power density in the reactor core using mesh tally. I have used the FM card & got the results. Instead of explicitly setting material number on FM card (61, in my...
22 January 2024 1,040 1 View
Do Germany, France and Italy maintain shariah index?
31 October 2023 5,355 2 View
Multi stage sampling for a nationwide study: We are planning a study comprised of assessing teaching (through classroom observation) and SLO (test) from public and private schools of six...
27 October 2023 7,493 5 View
Dear researchers and experts, I need some guidance about the identification key of fish. For references, the following is an attached example. More over, I also need identification keys of...
16 October 2023 1,508 3 View
I have five FASTA sequences
04 August 2023 6,125 1 View
I want to add my FASTA sequence. Can someone help me with it?
03 July 2023 6,969 2 View
Dear scientists, I am seeking assistance in understanding the signaling pathways of Auxins, Cytokinins, and Gibberellins in plants. If any plant expert could kindly provide me with a...
14 June 2023 7,880 3 View
I'm doing abaqus simulation on Metal to Composite single lap bolt joint plates with one side has encastre BC and load on the other end. Simulation completes but it bends at the point of the joint,...
29 April 2023 1,757 0 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
Molecular docking software/ websites?
02 August 2024 8,704 7 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View