I want my gene-sequenced data to dock so I can design a new in-silico drug. I really need help. If someone can do that for me or help me with then let me know.
How can I know the stationary phase of growth of several bacteria? We are aiming to collect exoproteome of several bacteria. We know that the ideal time to collect exoproteome is during the...
31 January 2021 4,528 10 View
Hi. I am new to Countour plots. I need help with the understanding of the following contour plot. If someone can explain, please.
10 November 2020 3,710 1 View
Dear Fellows I wanted to confirm if I can use spin columns of QIAquick PCR purification kits for DNA extraction. We finished our DNeasy columns but still have reagents. And we have extra QIAquick...
03 November 2020 6,960 3 View
Which method is suitable for transgenic plant development: In-planta transformation or tissue culture-based transformation?
13 September 2020 7,717 11 View
I want my biological sample to be analysed using TEM as we don't have that instrument. So can anyone suggest any institution or lab that provides cheap TEM experimental service in India.
10 August 2020 3,807 3 View
I am facing a problem to calculate the Slow and Fast light using Matlab. Can some share a simple code for Slow and Fast light. Thanks Xie Xie Danke.
13 June 2020 3,955 2 View
Hi, I am working on time-series data for the last 2 years. However, I have never used forecasting models. I want to enhance my statistical skills. As corona is all around us, I was thinking to...
29 March 2020 7,452 6 View
Linearity is pretty simple and obvious to guess. However, in some cases, the scenario gets totally different. IF you see the following attached graph, would you consider it linear association or...
15 January 2020 7,901 3 View
Hi, As the research field is really competitive, so what are the skills recommended for a Ph.D. student to learn while doing a Ph.D.? For example, I am doing a Ph.D. in environmental health...
13 January 2020 6,598 26 View
In time-series ecological study, do we take positive results (but statistically non-significant) serious enough to be generalized in the real population?
06 January 2020 4,236 9 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...
01 March 2021 3,703 3 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?
28 February 2021 1,577 1 View
Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?
28 February 2021 8,095 1 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...
27 February 2021 547 3 View
Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...
25 February 2021 6,969 3 View
I am learning how to use SPM for PET and PET-MR analysis, but I haven't found any great resources that explain how contrasts work. I have a series of PET images where our hypothesis argues that...
24 February 2021 1,534 2 View
I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...
24 February 2021 4,397 3 View