I would like to try to degas polyacrylamide gel 15 min before casting. But I don't have vacuum chamber or strong aspirator to put the gel in. Is there other way to degas the gel solution?
I think it is very possible to use a freeze-thaw cycling for this purpose. Just a small comment to the answer of Dr. Francisco Solano. This proposed time is it optimized? if so please refer me to any reference. This is because excesive sonication may cause degradation. Regards