Is there a way to improve the stability of the calcium alginate beads in alkaline pH?

I am working on heavy metal bioremediation using bacterial isolates. I have immobilized bacteria in 2% sodium alginate then cross-linked in 0.2M calcium chloride for 30 min. I have successfully...

04 January 2021 9,094 4 View

How to Strip off HSQ( Fox 16) after etching?

We are finding a hard time to strip of residual HSQ ( Fox 16) after using it as a mask in etching? We are coating 600 nm thick HSQ and writing with 3000 microC/cm^2 dose at 1 nA current. We are...

08 December 2020 5,452 2 View

How to measure the thickness of aluminium oxide made of aluminium coated nonmetal sample?

Aluminum coating has been done on nonmetal substrate. After coating the sample has brought in normal atmosphere ambient. In this condition oxide layer may have been formed. How to measure the...

11 July 2020 4,722 2 View

What is the reason for the low interaction of students in e-learning?

Through our dealings with students, we find a low rate of their interaction with electronic classes, and we note a decrease in the e-learning culture of some teachers. It is also difficult to...

30 April 2020 9,876 18 View

Formation of aggregates while synthesizing DOTAP-based liposomes?

Hello, I am writing this question to this online community in search of any help. I have been trying to synthesize DOTAP ( cationic ) and DOPE ( neutral) based liposomes using (20% of DOTAP in...

17 May 2019 6,233 2 View

What kind of tunneling losses can occur in Multijunction solar cell?

I am investigating different types of losses in multi junction solar cell. I came to know that, tunneling junction is a important factor for designing multi junction solar cell. What kind of...

13 April 2018 591 3 View

I would like to know about the reason for why Helium do not have triple point?

20 October 2017 4,019 2 View

Forest biomass/carbon stock calculation tool for Tier 1 IPCC ?

04 April 2017 6,542 3 View

Which technique from Elecrochemical analyser is used for electrodeposition??

I have electrochemical analyzer of CH instrument 608D.Can Electrodeposition of metal oxides on FTO or Cu are possible by using it ? And which technique from Elecrochemical analyses is used?

02 April 2017 4,691 3 View

How to separate copper sulfate from water?

14 September 2016 568 6 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View