Uday Jayaramaiah

3 Questions 1 Answers 0 Followers

Questions related from Uday Jayaramaiah

How does one detect PTM in bacterial surface proteins?

The protein was successfully cloned in pET19b, over expressed in E. coli BL21plys DE3 and purified in NI-NTA column using denaturating conditions. It is a bacterial surface protein can we detect PTM.

31 March 2015 8,811 3 View

Can we detect the function of Gram negative bacterial surface protein based on enzyme assay if so what methods can we adopt?

Can we detect the function of Gram negative bacterial surface protein based on enzyme assay if so what methods can we adopt

29 September 2014 8,652 1 View

How to dectect PTM In bacterial surface protein?

I want to test glycoslation and phosphorylation of the protein. What are the enrichment techniques of recombinant proteins?

27 June 2013 8,692 3 View