how to decrease the mortality of Lithium- Pilocarpine model?
Last few weeks, I tried the model of lithium pilocarpine model.
with 127mg/kg Lithium (24h before P)
1mg/kg Scopolamine methyl nitrate (30min before P)
and 30mg/kg P.
while the mortality is quite high. over 60%.
May I ask some sugguestion to decrease the mortality?
Besides, there is another question,
what is the difference between Scopolamine methyl nitrate and bromide? They are both used in papers.
Thank you for your reply. I have read this paper before, it does set a wonderful model for epilepsy. While the neuronal damage of this model seems not quite severe.@J. C. Tarafdar
In our group, we use the same protocol you described. For ending the SE after the desired time (2 or 4 hours, we use sodium thiopental (20 mg/kg). We usually decrease mortality by a more intensive care post SE: we place water in a low container inside the cage, and another with mashed banana + glicose. If the animal is too weak to eat and drink, we even give the mash once a day directly in its mouth, and use a Pasteur pipette to provide saline water. Still, mortality remains between 20 and 40 percent.
Read this paper, you may get insights;
Article Differences in Evolution of Epileptic Seizures and Topograph...