Dear Dadfar,

What is "autofluorescence"? What do you mean? Fluorescence without excitation? Something else? I'm sorry, but I have not seen this term before.

Please explain.

Dear Dabfar,

As I understand it, you are experimenting with physical objects, so use physical terms (not biological). Autofluorescence - a misnomer. Do not use Wikipedia, use primary sources.

As I understand it, you need to reduce the intensity of the luminescence of the film during the registration of the defect luminescence.

When registering SiV defect in CVD film, we proceed as follows:

1. Make sure that the film is well cleared. It is desirable to wash it in HCl acid, then with distilled water.

2. Do not register the spectrum of the sample surface, register for 5 - 10 microns below the surface (inside of the samples).

3. Correctly select the laser excitation. Figure – spectra of the same sample with excitation lasers with wavelength: 405, 470 and 640 nm.

Good luck