Hi,
I'm working on creating films and threads of different recombinant proteins, under different casting conditions. I was hoping to characterize them by FTIR and XRD to determine the presence and quantity of alpha helices and beta sheets.
Unfortunately, no one in my department has experience with proteins, deconvoluting FTIR peaks or interpret them. I was hoping someone here might have experience in this.
We are using a Bruker Tensor 37 with OPUS software. I'm attaching images of two IR's of 2 process in case it is helpful.
Thanks for any help it is greatly appreciated!
Hello,
at first sight, both spectra look very similar. There might be some frequency shift. You might want to record a reference and then compute differences. It is probably also not sufficient to use the peak assignment of OPUS as it can contain artifacts if a peak is not fully resolved or asymmetric. In any case, it is better to use a fit to the spectrum for a proper description of peaks.
There is a huge amount of literature available on analysing FTIR spectra of proteins:
You might want to look at:
http://jenalib.fli-leibniz.de/ImgLibDoc/ftir/IMAGE_FTIR.html
at first.
A brief look at literature also revealed this review:
https://www.researchgate.net/publication/15541719_The_Use_and_Misuse_of_FTIR_Spectroscopy_in_the_Determination_of_Protein_Structure
However, I am not an expert in FTIR spectroscopy of proteins, since I mainly perform FTIR spectroscopy of aqueous solutions.
All the best,
Hendrik
http://jenalib.fli-leibniz.de/ImgLibDoc/ftir/IMAGE_FTIR.html
Article The Use and Misuse of FTIR Spectroscopy in the Determination...
https://www.researchgate.net/post/How_can_one_quantify_the_secondary_structure_of_protein_by_FTIR
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