What are the possible effects of COVID -19 Vaccine/s on the persons already recovered from the disease?

Persons recovered from COVID -19 are having antibodies against the virus inside their bodies. They are having T cells already sensitized to the antigens of the virus. If they are vaccinated (may...

12 February 2021 4,106 22 View

Diabetes control: What is most important - treatment, food or following of designed lifestyle? Is it possible to follow all of them? If yes, how?

Diabetes control: What is most important - treatment, food or following of designed lifestyle? Is it possible to follow all of them? If yes, how?

20 April 2020 4,943 39 View

"Molecular Pathological Epidemiology" concept may change the ongoing disease treatment system drastically - what is your idea?

Molecular pathological epidemiology correlates individual genetic make up, different aspects of lifestyle followed, living environment etc. with disease. Do you think this new concept will...

19 November 2018 3,047 15 View

Scientists has only liable to perform research, they are not to consider the effects of their discoveries on the society. What is your comment ?

All the dangerous mass killing gases, G.M. Bacteria and Viruses and other devices, weapons etc. are developed by some brilliant scientists. The politicians just only use these. Even today, such...

22 May 2018 5,114 24 View

Why reviewers of the articles are not paid by the journal authorities?

Actual reviewing of an article requires clear and updated knowledge on the subject as well as about the stage of contemporary research on the topic of article. It is a tusk of experts. But...

18 February 2018 8,928 85 View

How quality of research is affected by the data analytical methods used?

In many very good articles even of 1990s, the Average and Standard Error of data were published. Presently, no journal will send any article to the reviewers without having analysis of data...

07 February 2018 9,133 16 View

Software models are developed to replace use of animals in research. How much they are effective? What are the problems in using of this technology?

Many small animals are sacrificed for scientific research. Though they are bred separately for such purpose, but the pain received by the animals during experiment and loss of resources for such...

01 February 2018 9,812 25 View

Do you think all the researches are based on collection and analysis of data and then coming to a conclusion?

A debate has been initiated by one of our learned scientist of Research Gate that all the researches should be based on scientifically collection of data, their scientific analysis and...

17 January 2018 2,909 35 View

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We use Microwave ovens to save time in preparing foods, mainly fast foods. Please read the mechanism of generation of the heat. Microwave heating is the process in which a high-frequency...

03 January 2018 7,692 44 View

Why teaching by video conferencing in the colleges is not advocated?

The lecture of one professor can be followed by thousands of students of colleges together on a large screen through video conference. It can be arranged in all the colleges of a University or...

13 December 2017 6,630 25 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View