I want to measure the soluble Pi concentrations in rice roots and leaves. I need a standard curve with varying concentrations of KH2PO4 in order to calculate the pi concentration. I am confused and do not know how exactly I can generate a standard curve for my calculations. I would appreciate any suggestions in this regard.
Many thanks.
First, explain what is " soluble Pi concentrations in rice roots and leaves ." Commonly, Pi means a total phosphate. What is your procedure? At low pH roots and leaves undergo hydrolysis releasing more Pi. At basic pH, the main species of Pi is phosphate, PO4(2-), which form almost insoluble complexes with transition metals. So, [Pi] might be dependent on pH of aqueous solution.
Second, how do you measure Pi?
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