What is the impact of Washback in teaching a language or any other subject?

The importance of washback in teaching and learning the language through assessment

15 November 2020 3,559 4 View

How can we use 'assemblage theory' by multi-disciplines for designing a better children environment?

A research group is conducting a study across several cultures to investigate insights about issues in the environment of school-age children (6-12). The purpose is to explore practitioners'...

05 August 2020 1,967 5 View

Can you help in the two-minute survey about nightlife atmospheres in historical places?

19 July 2020 9,584 15 View

Load deflection curve in abaqus?

Ihahve modeled RC beam in abaqus, but the load decrease extremely after the maximum is reached, what could be the problem?

03 May 2020 2,566 8 View

What is the difference between connector type axial and friction in abaqus to define a bond between concrete and steel?

what is the best way to simulate the bond between steel and concrete if i will use connector, what is the difference between connector type axial and friction how i can define shear stress...

25 April 2020 5,880 3 View

Can I use anhydrous ammonium phosphate in oil and grease extraction?

hi, can I use anhydrous ammonium phosphate instead of anhydrous sodium sulfate in oil and grease extraction?

23 March 2020 9,987 3 View

Is test of significance(p value) necessary in some researchs??

hi, i'm writing a research about decolorization of waste water by fungi. the results obtained by % decolorization. Is it necessary to include test of significance??

29 December 2018 7,535 3 View

What's the typical active area for anode and cathode for EIS test?

EIS, Active areas of Anode, Active area of cathode, SOFC

16 November 2018 3,137 4 View

Can you help me ,i need resent researches on orthogonality in Banach Space?

I begin anew research on this topic

09 September 2018 9,062 5 View

How to do premeabliztion for LR-white resin section on formvar coated grid for IF ?

I'm trying to do Immunofluorescence staining for LR-white resin section on formvar coated nickel grids. I have problem in antibodies penetration. I've tried to use 50mM glycine followed by 0.1%...

27 May 2018 7,495 2 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View