Hello
I am looking for an automated or semi-automated way to count bristles on Drosophila Thorax. I have the MorphoJ but what is the best way to generate TSP files?
I would like to create a small guide containing the meanings of the names .. Thanks to everyone who cooperates with me in achieving it.
03 March 2021 5,266 4 View
I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...
03 March 2021 4,116 4 View
Actually, I am running cfd simulation for a heat exchanger which has two fluids one hot and other one is cold fluid. there is a solid domain between them, which I removed and instead I have used...
01 March 2021 9,537 2 View
I am simulating Heat Exchanger. I have tried and tested all the methods to resolve this issue from the internet like refining mesh, improving skewness and orthogonal quality ,extending outlet to...
01 March 2021 6,985 3 View
I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...
01 March 2021 360 7 View
Should I mesh the solid domain ? or should I use wall thickness? or Shell conduction? for Heat Transfer between two fluids of a Heat Exchanger. what is recommended?
01 March 2021 9,785 3 View
I have a virtual machine. My host Pc specs are not that good thats why i went for virtual servers. so basically my host pc has 8gb of ram with nvidia quadro k2200 4Gb DDR5 GPU ,processor intel...
01 March 2021 8,415 1 View
Preference in terms of the scientific aspect
28 February 2021 796 2 View
Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.
27 February 2021 9,356 3 View
I build a model of 4 regression equations (probit and OLS) in R studio. I need to take the weights of observations into consideration. While it is simple to do so for every regression equation,...
27 February 2021 1,233 1 View
Does anyone have experience with collecting ECG data on children with autism? How was your experience? I'm interested in the tolerance levels for kids to have wires attached to their chests. What...
02 February 2021 5,850 3 View
I work with insect primary cells, which I let grow in TC100 insect medium. For fluorescence measurements I have to discard medium (because of its fluorescence properties) and give a saline...
28 January 2021 3,867 1 View
On inducing the mouse bone marrow cells with X compound, there is a change in cells morphology and characteristics. In that case how to gate these cells, as these cells are showing suppressive...
27 January 2021 608 2 View
I am working on solving an optimization problem with two objectives by using neuroevolution. I use NEAT to evolve solutions which need to satisfy objective A and objective B. I tried different...
27 January 2021 4,875 3 View
Based on the many publications from several decades, we came across many classifications of Insects. Among them which one is more updated and approved classification that can be used for research,...
05 January 2021 3,668 9 View
Hi everyone, So these are images of IPSCs 2 days post passaging/splitting. I noticed that the overall morphology of colonies are not compact or round and without distinct border. However, at...
28 December 2020 3,503 3 View
I need to design new oligonucleotides that can be used for gRNa/Cas9. The oligonucleotides need to target a gene from melangoster drosophila. Where do I start? How to find the gene to knock...
15 December 2020 4,729 3 View
I have designed an SPR based PCF sensor, which has a TiO2 and Gold coating. A reviewer has asked for the characterizations (e.g. SEM, TEM) to study the surface morphology. The reviewer has also...
06 December 2020 7,210 3 View
If I want to isolate Tyrophagus entomophagus by selective media, as I know they can grow on molds of stored grains,fruits, cheese. Or even in beehives debris. But how can I got only one species...
30 November 2020 9,087 3 View
In simulation practice/training, are there set standards for monitoring quality of a resuscitation attempt? beyond rate/depth of compressions and time off chest?
23 November 2020 5,182 12 View