When I was doing a blast search of a nucleotide sequence I got a message "Message ID#30 Error: Subject string not found in the CGI context " How can we make the sequence in CGI context? Any comments..
HI..
It sounds like you are trying to perform a BLAST search using the NCBI BLAST service, and you are encountering an error message that says "Subject string not found in the CGI context". This error can occur for a variety of reasons, but it typically indicates that there is a problem with the input sequence that you are trying to search against.
To correct this error, you will need to ensure that the input sequence is in the correct format and meets the requirements for the BLAST search. Here are some things you can try:
If you have checked the input sequence, database, and parameters, and you are still encountering the error message, you may need to contact the NCBI BLAST help desk for further assistance. They should be able to help you troubleshoot the problem and suggest a solution.
Regards .
Hi
Thanks for your reply. Actually I have used a plasmid sequence which is designed by another lab.
1. Yes it is complete sequence without errors.
2.It is plasmid sequence, So I have used Nucleotide BLAST tool.
I will try it again.
Thanks for your suggestions.
- Badges
- Science topic
- Similar topics
- Biological Science
- Biota
- Eukaryota
- Fungi