How to convert your health control to 1.00 from 1.05 in case of fold change or gene expression analysis?

More Najifa Yeasmin's questions See All

Why am I getting this kind of curve? Previously it was fine.Now it does not come at all trying after several times.what shoud I do?

I am using sybr green mastermix and my cDNA quantity is okay. Still not getting amplification curve both for expected gene and internal control (GAPDH)

20 April 2024 9,896 5 View

Can mirNeasy serum/plasma kit(qiagen) be used to extract long non coding RNAs from plasma?

I am doing a thesis on estimation of plasma levels of several long non coding RNAs in Hepatocellular carcinoma patients. mirNeasy serum/plasma kit manual says total RNA purification kit it is. Any...

30 August 2023 221 0 View

In case of asymmetric supercapacitor which electrode will act as working electrode?

Since in asymmetric supercapacitor both the electrodes are different, its a bit confusing which electrode will act as working electrode and which electrode will act as counter as well as reference...

26 December 2022 7,805 4 View

How to calculate the average conjugation length of a polymer from FTIR?

Kindly update on how to calculate the average conjugation length of a polymer using the intensity ratio from FTIR spectra.

26 January 2022 6,712 6 View

Why we measure the absorbance of light in different wavelength in UV Spectrophotometry? why 750nm in TPC and why 415nm in TFC?

I want to know, what's the cause that we are measuring the absorbance at different wavelength? why not the same? what would be if I used a same wavelength for all the assays related to it.

18 December 2021 3,353 5 View

Is hydrogen bond spontaneous or requires incubation time?

Hello, In MIP sensors, monomer and target molecules are mixed together, and electropolymerization initiates polymerization to create molecularly imprinted polymers (MIP). The literature says a...

23 August 2021 3,546 3 View

How to control the over oxidation of pyrrole?

I am preparing molecularly imprinted polymer (MIP) with electropolymerized polypyrrole. I am taking advantage of overoxidized pyrrole (by applying potential) for template removal. I want to...

17 April 2021 404 2 View

Vinyl monomer and aniline copolymerization?

I want to co-polymerize vinyl monomer and aniline monomer. Is it possible? Thanks.

14 April 2021 7,169 4 View

In which cases median is used instead of mean?

Normally we use mean value to express sociodemographic characteristics of respondents ( e.g. average age of rice farmers). In few articles, median was used instead of mean. How can I...

01 December 2020 8,241 8 View

What is the difference between surface controlled and diffusion-controlled processes in cyclic voltammetry?

In the diffusion-controlled process, peak currents are linearly proportional to the square root of the scan rate. In surface controlled (also known as an adsorption-controlled) process, peak...

01 August 2020 7,129 3 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

How to back transform the results generated from analyses using log transformed with In(X+1) data?

I am conducting my analysis using SPSS. I log transformed my data using In(X+1) as my data contain zero values. However, when I want to back transform the regression coefficients generated from my...

31 July 2024 7,860 3 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

How to prediction 2D to 3D aptamer?

Hello, I have a little problem. Well, right now I only have the DNA aptamer sequence. I went to see the 2D model form with DNA folding. After that, I used RNA Composer to simulate in 3D and used...

30 July 2024 6,794 0 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

Are these cassettes suitable for expressing PETase mutant in E. coli?

I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...

28 July 2024 7,559 1 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 10 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?

27 July 2024 6,920 1 View

If my gene of interest has high GC content can it be problematic in sequencing? What kind of error is expected with GC rich gene sequences??

Gene sequencing related trouble shooting

25 July 2024 4,149 2 View

Libor Krásný

Yes. This will help visualize the trends/fold differences.

If you set the healthy control group value (1.05) as 1, you then calculate the value for your disease group.

An example:

healthy group: original value 1.05

disease group: original value 2.65 (I am just making this value up as an example)

So, when 1.05 corresponds to 2.65, you then calculate what corresponds to 1: 2.65/1.05=2.52381.

Najifa Yeasmin

Thanks a lot