Najifa Yeasmin

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Questions related from Najifa Yeasmin

How to convert your health control to 1.00 from 1.05 in case of fold change or gene expression analysis?

I am seeing expression of some gene into disease group comparing to healthy control group.Mean value of healthy couldn’t come exact 1.00...It is now 1.05... Is there any method for transformation...

19 September 2024 3,940 2 View

Why am I getting this kind of curve? Previously it was fine.Now it does not come at all trying after several times.what shoud I do?

I am using sybr green mastermix and my cDNA quantity is okay. Still not getting amplification curve both for expected gene and internal control (GAPDH)

21 April 2024 9,931 5 View

Can mirNeasy serum/plasma kit(qiagen) be used to extract long non coding RNAs from plasma?

I am doing a thesis on estimation of plasma levels of several long non coding RNAs in Hepatocellular carcinoma patients. mirNeasy serum/plasma kit manual says total RNA purification kit it is. Any...

31 August 2023 247 0 View