Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Can anyone suggest a commercial MAT kit to detect Toxoplasma gondii antibodies in animals?

18 January 2021 5,438 2 View

What is the purpose of internal control and extraction control in q PCR procedure?

Is extraction control also add to the qPCR mixture with the sample or does it run separately? Can anyone please explain their importance and how to use them in q PCR protocol? Thank you.!

23 November 2020 314 3 View

Can anyone explain the difference between recombinant proteins vs recombinant chimeric proteins?

Difference and advantages and disadvantages of using recombinant proteins versus chimeric proteins as antigens in serological tests

28 September 2020 5,307 3 View

Is there any possibility to detect both IgG and IgM antibodies in the same ELISA plate?

I am looking forward to developing an ELISA which can catch both anti-Toxoplasma IgG and IgM antibodies in Marsupials. Has anyone have any thoughts about detecting two different antibodies ( IgG...

24 July 2020 4,193 3 View

What are the software/tools available for SEM image analysis of bacterial biofilms?

Dear Researchers: Greetings! I am interested in learning electron microscopy image analysis of bacterial biofilms, mainly using open-source software. I have been using basic functions and tool...

28 May 2020 5,572 4 View

The condition has length > 1 in ifelse in r?

When the condition has length > 1 in ifelse in r and there are more than 2 statements how to use ifelse? I have a data set wherre I want to categorise people in to categories using sveveral...

04 March 2020 4,811 6 View

When we are using already available records, in a retrospective cohort study the sample size calculation to be done in which formula?

I have started a study of record available at clinic collected years back.So it seems to be a retrospective cohort study.But when calculating sample size I feel like it to be done in normal cross...

12 August 2019 866 3 View

Can machine learning methods to model time-to-event data?

08 May 2019 7,631 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Can I download BibExcel for MacOs?

Hi everyone, I would like to ask how I can download BibExcel software for MacOs? There are some links which are supported by Windows but I could not find it for Mac. or is there any other software...

01 March 2021 652 1 View

How to arrange coordinates and extract climate dataset out of MPI-ESM-MR netcdf files?

Have a nice day everyone, I'm stuck in extracting datasets out of MPI-ESM-MR netcdf files because the latitude ranges from -3*10^6 to 3*10^6 and longitude ranges -5,9*10^6 to 5,9*10^6. Plus, the...

28 February 2021 912 3 View