How to convert RFU values (in thousands ) to picomolar range ?

03 May 2022 0 579 Report

Data is recorded for different concentration and RFU values are obtained . However, to calculate pm range for unknown values these RFU should be converted to pm range. This is GLP-1 elisa carried out and data is recorded at Fluorostar Optima mentioned below

2pm-5081923

5pm- 5257968

10pm- 63451179

20pm- 81101492

50pm- 158142953

100pm- 425828186

Any help would be really appreciated.

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