I am working on RT-qPCR. Using Bio-rad CFX manager to analyze the gene expression data. Attached file is sample of my work
Thanks
The first few qPCR runs the primers worked and I was getting signals in my samples/positive controls. However, now when I run another qPCR with the same primers I get no signal. I've tried using a...
17 October 2022 746 5 View
I've got a Roche LightCycler that I use to run my qPCRs. It used to take 1h 37 min to run a qPCR but now it take 1h 45 min using the same exact settings. Could the problem be that the machine is...
17 October 2022 9,072 3 View
XRD data sheet standard JCPDS manual
01 February 2020 7,466 3 View
I am looking for EEG dataset for anesthesia, please where can I find such dataset?
10 November 2019 6,786 3 View
I’ve found out several clustering algorithms such as chameleon or even Spectral Clustering. its work to converting data to a weighted. KNN graph based on the distance between points. I'd like to...
09 October 2019 6,802 4 View
Scientific Workflow scheduling in cloud computing and I need suggestions on the gaps in this domain. The gaps that would need to be directions for future works?
08 September 2019 4,527 2 View
HI RG colleagues, some statistics' comments believe that the Baron And Kenny 1986 mediation method out of date. they suggested new effective approaches in term of testing the mediating variable.
07 August 2019 4,191 15 View
The advantages of this index are evident. The h-index can obviously be applied to any set of papers. Since the h-index cannot exceed the umber of underlying papers, many authors...
06 July 2019 6,316 3 View
The number required by the journal for the publication of articles does not exceed six thousand words, while the total words of article nearly 8000.. Should I follow the journal instructions in...
06 July 2019 7,211 33 View
Although I realize the success made using Python for different aspects of ANN and deep neural networks. However, I am a MATLAB user. Is it worth it to learn Python for that purpose? or is MATLAB...
04 May 2019 8,500 2 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View
Hello. I have the geometry of a blade in CAD file (stp) and I want to prepare the blade for meshing with turboGrid. I must import this file into designModeler and then transfer to the...
27 July 2024 356 3 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View
Gene sequencing related trouble shooting
25 July 2024 4,149 2 View
I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?
23 July 2024 1,353 4 View