What cytokines and small molecule compounds need to be supplemented in mouse pancreatic organoid medium? What is the specific concentration? Thank you!
As described in the Multifractal detrended fluctuation analysis (MFDFA) algorithm, it at first calculates the profile of the time series, and then other steps are operated on the profile....
05 August 2024 9,366 2 View
Hi, Could someone explain the primary differences between deep-seated landslides and slope destabilization? In particular, definition and characteristics, mechanisms and triggering factors,...
02 August 2024 4,212 2 View
How long it takes for arbuscular mycorrhiza to establish and produce benefits under experimental conditions?
25 July 2024 5,208 2 View
I want to study the thermal properties of a mixed system which is constructed by virtual crystal approximation in VASP. When I try to run the ab initio Molecular Dynamics of this system in VASP, I...
19 July 2024 6,569 3 View
If I want to invent my own hypothesis testing method, where should I get started ?
15 July 2024 5,376 5 View
I am currently working on a research paper focused on the control of Connected and Autonomous Vehicles (CAVs) utilizing multi-agent reinforcement learning methods. At this stage, I am seeking a...
14 July 2024 2,620 2 View
I've earned 1 best paper award and 4 best oral presentation awards. What should I do next to elevate my academic capabilities to the next level ?
14 July 2024 6,071 5 View
I read several information security books. How do I start writing anti-virus softwares ?
13 July 2024 8,180 1 View
I suspect my cells are contaminated with mycoplasma. I fixed the cells with 4% PFA and stained them with DAPI. Below is the image I obtained. I don't observe the typical small, rounded DAPI foci...
11 July 2024 7,786 3 View
During the heavy warm rolling process, austenite undergoes dynamic recrystallization (either DDRX or CDRX), and then transforms into martensite upon quenching. Is there a way to distinguish from...
11 July 2024 7,602 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Do experiments on behalf of Our Business: Laboratory animal rearing Animal modeling Experimental animal behavior detection, image ultrasonic detection, electrophysiology platform Organoid...
05 August 2024 5,829 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View