Why is ccdb toxin not killing susceptible strains of E.coli?

Hi hive, We are trying to use ccdb as a counter selection marker. We are using the cassette from this paper, which should be highly expressed: A new method to customize protein expression vectors...

22 June 2020 5,084 5 View

Appropriate statistical test for within-group comparisons of questionnaires?

Dear colleagues, I am trying to determine the appropriate statistical test for my problem. I have read several discussions on the topic here, however was unable to extract an answer, and so I...

03 May 2020 6,281 10 View

How to interpret transition states whose Gibbs-energies are higher than the Gibbs-energies of their corresponding ground states?

I am performing DFT-calculations (gamess-us) on a reaction: ground state 1 -> transitition state -> ground state 2. (in short: gs1 -> ts -> gs2.) ts is only 0.3 kcal/mol higher in...

16 February 2020 2,261 1 View

Open datasets for Machine Learning in geotechnics / engineering geology?

Machine learning has proven to be very successfull in many disciplines and the general development is surely furthered by openly accesible algorithms and datasets for the whole community to work...

23 October 2019 6,941 19 View

Is it possible to produce a device that can project screen out of the mind in physical - how is the theory of that?

Can there exist societysystem including projections of pictures and video frames out of thought into the physical reality? Is this possible for nature of human beeings or has this to be technical...

30 May 2019 2,426 5 View

Secret of neural stem cells?

Hi! I can not find a list of growth factors that secrete neuronal stem cells. Usually everyone writes about neurotrophins. But I think there should be other factors ( fgf, egf, igf, vegf)?

12 November 2018 9,084 2 View

Hydrophilicity of PDMS due to treatment with Piranha and KOH. Treatment time?

Apart from plasma activation, PDMS can be made hydrophilic by treating it with Piranha solution (and maybe KOH in an elaborate approach). I found two papers reporting this effect. Interestingly,...

18 April 2017 1,857 4 View

Could anybody provide me with a table for the redshift and distance of at least 30 galaxies within 1 billion light-years?

Additionally details of measurement for the distance would be appreciated, but is not mandatory.

01 November 2012 2,944 2 View

I would like to hint to my projects related?

Please have a look on my publications Georg Oesterdiekhoff For example Child and ancient man American Journal of Psychology 2016

01 January 1970 8,102 5 View

Has there ever been a scientific approach to increase awareness as a combination of reverse engineering entropy?

Hello, we all know entropy from physics and complexity of information, so my question is about the possiblity to reorder information zu a certain more ordered state of the past by human and...

01 January 1970 1,050 1 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Logistical regression using SPSS for a dichotomous dependent variable, with an independent variable of 1, 2, 5 and 10 seconds?

I have an experiment with 28 participants The independent variable is the times 1, 2, 5, and 10 used as the cross hairs during a task These 4 times were tested using 4 different blocks, each...

03 March 2021 9,692 2 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

No title

01 March 2021 3,905 5 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

How can we simulate an hyperelastic-viscoelastic behavor with Ansys APDL?

I am working on modeling and simulation of biomecanical material behaviour, I have succeded on simulating skin using anisotropic hyperelastic material, on APDL and in our lab's finite element...

28 February 2021 552 3 View