I have a min-prepped plasmid (pBAD) and i want to concentrate it for sequencing. Is there any simple method to do that like ethanol precipitation or using N2? If so please explain the procedure.
Depending on the volume to evaporate, speed vac is very straight forward (evaporation under vacuum). Otherwise ethanol precipitation (for protocol read any molecular biology book like Maniatis for instance) or evaporation under N2 are indeed an alternatives.
Dr. Dominique Liger is rite. otherthan these methods you can also use any PCR purification kit with low elution volume. This procedure also get the purified plasmid.