Ashkan Shahsavan

3 Questions 4 Answers 0 Followers

Questions related from Ashkan Shahsavan

How to set up an affinity chromatography column using syringe?

Is it possible to make an Ni-NTA affinity column with a syringe? if so how? My column is blocked and no solution is eluted from it. So i can't purify my His-tagged protein using this column. I...

11 November 2018 7,785 4 View

How to concentrate Plasmid DNA?

I have a min-prepped plasmid (pBAD) and i want to concentrate it for sequencing. Is there any simple method to do that like ethanol precipitation or using N2? If so please explain the...

09 September 2018 7,776 4 View

Why don't I get any colony after DNA transformation?

I transformed a plasmid DNA into my own made competent cells (DH5a) and i got no colonies neither in my sample plate nor in positive control. I prepared competent cells using following protocol :...

09 September 2018 8,745 8 View