Hi,

I use decellularize tissue i,e where I use tissue with low or no cell. In order to check the DNA integrity by agarose gel electrophoresis, (as the DNA content of cell is supposedly low) I had to load a very high volume e.g 50 -100 microlitre corresponding to 250 ng. 

When I tried to use ethanol or isoproanol based concentration, I lost almost all of the small amount of DNA I had.

So, I thought to concentrate the DNA by keeping the DNA sample at 37-40 deg C overnight. It worked and the sample volume was reduced but then on running the gel, I found the high base pair of DNA mainly, which are supposed to be eliminated as my samples are normally treated with DNAse. I expected low base pair less than 200 base pair to prove my decellularization efficiency.

Can anybody suggest what went wrong- the concentration process or the DNAse treatment had failed?

Cheers

Debashish