What is the chemical composition of Cucumis melo roots?

I have found an old pharmaceutical research from 1833 in which emetic power of Cucumis melo roots was confirmed. I cannot find data about chemical constituents of the Cucumis melo roots. Their...

30 October 2020 3,633 3 View

Can I use two different randomly chosen samples to make binary logistic regression and present it in the journal research paper ?

R programming language I am considering if is it appropriate to use two different randomly chosen samples coming from one huge database to proceed two logistic regressions separately on the same...

21 July 2020 3,737 5 View

Does anyone have any materials in this topic: Reverse Vending Machines RVMs?/plastic recycling system?

Hello there! I'm trying to write an article about reverse vending machines systems. I would like to deepen the study with statistics and international comparisons or reports. Does anyone have any...

20 May 2019 5,389 2 View

How to model PV system using the weather data from NASA POWER project?

I am trying to evaluate solar (PV) potential for various areas in the world with use of daily data. The NASA POWER tool (https://power.larc.nasa.gov/data-access-viewer/) which I consider using,...

01 January 2019 1,773 1 View

How can I model a graphene layers (films) in Sentaurus TCAD?

I know that the similar topic was discussed on this forum. Unfortunately, link with solution is inactive. So, maybe anybody of you have information about graphene from this link...

11 January 2017 1,476 3 View

Why is it so that 99% of soil bacterial strains cannot be culture-grown in the lab?

The recent success of teixobactin by Lewis and Epstein (of Northeastern/Boston) was brought by their ability to tap into the "unculturable" bacterial strains. Turns out 99% of strains have been...

07 January 2015 2,671 14 View

Who is interested in helping to compile and map a global fault zone permeability database?

I am compiling global database on fault zone permeability, in rocks, any depth. Looking for hard to find data: tunnels (engineering reports), anything not published in journal papers or not in...

22 December 2014 3,409 6 View

Strange result of genetic cross

I am working on eye colour mutants of house cricket (white - W and yellow - Y, wild type, black - B). I conducted series of genetical crosses to check how observed traits (genes) are inherited. I...

01 January 1970 3,084 3 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View