What is the simplest way of stabilizing the concentration of Hydrogen Peroxide in an aqueous solution? I seek the easiest and let's say "home-made" method.
Thank you.
Hi Jacek Matys, a common and very easy method of stabilizing aqueous hydrogen peroxide solution is the addition of small amounts of phosphoric acid.
Hello Frank T. Edelmann. Thank you very much for your answer. Do you know maybe how many milliliters of 85% phosphoric acid is needed to stabilize a 6% solution of hydrogen peroxide?
I think the simplest way to stabilize the concentration of hydrogen peroxide in an aqueous solution is to always use cooling with prepared solutions and keep cooling constantly.
Jacek Matys
Hi Jacek Matys, I don't know for sure, but a standard 3% stabilized hydrogen peroxide solution contains 5 g of 10% phosphoric acid per liter. Sometimes you also find in the nliterature that the pH should be ca. 4,2. Thus (without guarantee) I would estimate that 1-2 mL of 85% phosphoric acid per liter should be sufficient. Apparently this will stabilize the H2O2 solution for ca. 1 year when it is kept cold.
The thermal decay of hydrogen peroxide in room temperature is not that much, so a significant change in its concentration during an experiment should not be happen. However, if you want to keep it's stock solution concentration, you must save it in the fridge.
I fully agree with Firas A. Al-Lolage that the short-term stability of low-concentrated H2O2 should be no problem.
when you want to prepare 1 percent solution for Covid treatment
how do you stabilize for more time
Dear Rama Rao Tantravahi please see this useful link entitled "A Study Monitoring the Hydrogen Peroxide Stability for Shelf-Life Determination"
https://www.texwipe.com/Content/Images/uploaded/documents/Technical-Data/TechNote_Hydrogen_Peroxide_Shelf_Life.pdf
According to this article highly diluted hydrogen peroxide solutions have a shelf-life of up to three year without any stabilizer.
We are using it for oxidative stress in shsy5y cell line but getting different result of the damage in each experiment.
Any idea why it is happening?
We dilute 30% solution in water and use immediatly
Thanks
Common stabilizers: https://www.waterboards.ca.gov/losangeles/board_decisions/adopted_orders/WDR_Update/Sodiumcitrate,sodiummalonate,andsodiumphytate-OxidantActivators.pdf
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