Why FTIR can not detect bases of nucleic acid in extracellular polymeric substances (EPS)?

I characterized functional groups of EPS using FTIR. Goups of sugars and proteins (e.g., -OH, amide, -COOH) as well as phosphate group of nucleic acid were readily detected. However, peaks of...

05 February 2021 4,405 1 View

Why my BOC-protected compounds got deprotected during evaporation?

I purified my Boc-protected molecule using HPLC. The eluent is ACN/H2O/(0.05% TFA) . After I evaporated the solvent (65 oC), the product was collect as the MS result showed ~20% of the product was...

14 January 2021 5,487 5 View

Who can tell me the easily acquired Pb hyperaccumulator?

The common used Pb hyperaccumulators in the experiment, which and can buy from the business. Thank you!

14 January 2021 460 3 View

Does anybody have the recipe for making a home made R electroporation buffer for Neon transfection systems?

I tried the recipes below in two cells lines: 1.DPBS 2.DPBS+MgCl2(1mM) 3.DPBS+Sucrose(250mM) 4.DPBS+MgCl2(1mM)+Sucrose(250mM) 5.R Buffer (Neon) the results showed that R buffer is the best one in...

06 January 2021 6,776 1 View

What's the criterion of the Discriminant Validity between dimensions of second-order variable?

Hello All, I am doing a study that includes two constructs, using PLS-SEM. Based on previous literature, those two constructs can be included in the same category. Considering the discriminant...

05 November 2020 1,295 2 View

Is there a technique to implant a cannula for repeated CSF sampling?

We need to collect CSF samples repeatedly from the 4th or lateral ventricle of rats. Does anyone know of a protocol for implanting a cannula to be able to do this? Or even a protocol for this...

26 October 2020 5,804 3 View

Are there any structural differences between sucrosic dolomite and saddle dolomite?

Sucrosic and saddle dolomite are known to be different petrographically. However, are they different in structure, e.g., under XRD or TEM?

20 September 2020 2,495 2 View

Why simple site-saturation mutagenesis is not successful?

We are doing site-saturation mutagenesis of LeuDH. But when we are mutating Q to G, the result of the sequence is always wrong, only a small fragment is the same to our templet plasmid. I have...

15 September 2020 9,201 8 View

Why is there extra bands on restriction digest gel after mini prepping transformed bacteria?

Hello I recently transformed bacteria with my vector and insert ligated construct. In order to check the presence of both the vector and insert, I mini prepped 20 bacterial colonies and did...

14 September 2020 7,465 4 View

For Ultrafast Transient Absorption Spectrometer: How to choose a spectrometer?

hi,we want to build an Ultrafast Transient Absorption Spectrometer system, how to choose a spectrometer? We already have femtosecond lasers system (coherent) ,pump probe system(home built), white...

10 September 2020 4,693 3 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

How to find the mode of classified ocean colour images?

Hello, I have classified 10 S3 OLCI images from the same area (althouth not the same size and/or quadrant) and I would like to do a final image using the mode of the pixel of these 10 images. I...

01 March 2021 9,874 3 View

How to threshold an image despite bright lighting, using ImageJ/Fiji?

Hi everyone, I am using Fiji to determine the percentage of the image covered by leaves, branches, or trunk, but i've encountered some difficulty when the sun hits parts of my image like the one...

27 February 2021 1,194 3 View

Trouble with Charting with Google Earth Engine (Technical)?

Hello. I have a problem with the charting in google earth engine. I created an image collection by combining 5 image collections of five parameters and using ee.Join.inner ( ) and .apply ( )...

26 February 2021 7,084 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View