[SAP2000 related] I wonder why there is no any effect in the analysis result when I change the different parameters while modeling a frame structure?

(i) I have modeled a simple 2D frame structure (2 columns and 1 beam). I would like to see the variation in the result by varying the different parameters such as, sizes of confinement bars,...

06 October 2020 9,496 5 View

If a word file is shared with a person via Google Docs, can the recipient have access to the other files?

We can share and edit 'word' files online via Google Docs. It is an easy and convenient way of creating, sharing and editing the same document online between the concerned users. However, my...

22 September 2020 675 3 View

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I am working with new fish cell lines especially from cold-water fishes, so how can we choose the best program and process to get cells with higher viability and transfection efficiency?...

16 September 2020 9,574 2 View

Which gRNA designing tool for Crispr experiment can be effective for the gene knock out experiments in fish cell lines specially like Atlantic Salmon?

Any suggestions for critical things to be considered for the fish cell line as it is for cold water fish which is completely different than other mammalian cell lines.

14 August 2020 7,083 2 View

Understanding the equation [C26.11-8, ASCE 7-16/SEI]?

While going through the ASCE 7-16, I encountered with the equation C26.11-8 under chapter 26 'Wind loads: General Requirements'. fn1=150/h (ft) ------------- Equation...

01 March 2020 1,643 3 View

Is it possible to determine the bandgap energy of CuO nanoparticles from their UV-vis absorbance spectra?

I could not find a sharp absorption peak in the UV-vis absorbance spectra of CuO nanoparticles dispersed in pure ethanol solvent. Hence, the calculation of bandgap energy by Tauc Plot could not be...

12 February 2020 9,241 5 View

Understanding the modulus of elasticity (Young's, Secant, Tangential)?

Dear all, As per my understanding, we use Young's modulus of elasticity of the material when it is under the linear range and we use secant/tangential modulus of elasticity when it is in the...

22 December 2019 3,613 3 View

Why microbial community structure didn't increase with soil organic matter ?

As SOM is driving factor for microbes, why didn't they increase with SOM ?

13 September 2019 2,845 5 View

What are the reason for non amplification of KASP markers during PCR?

I run many KASP marker of FHB resistance in wheat NILs.But for one of these marker one marker is only 50% amplified.However, other went well.

15 March 2018 790 3 View

How can we get the bearing capacity of rock and soil?

for shallow foundation and deep foundation.

06 August 2017 5,650 4 View

Heat exchanger with liquid Nitrogen as coolant?

I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...

01 March 2021 6,450 5 View

How to get/count refractive index in gasses?

20 February 2021 8,202 6 View

Can one use just 250 micron sieve & still get a good number of mycorrhizal spores from the soil?

In a situation where one cannot get the appropriate & separate levels of sieve for mycorrhizal survey work, can just one level of sieve give good results & spore counts?

13 February 2021 3,760 2 View

How to make Fast Green FCF from powder?

Hi everyone We need Fast Green to electroporate plasmid into mice embryos, therefore we simply need it to see some color in the liquid we are electroporating so that electroporation is more...

07 February 2021 1,671 2 View

Which fungicide are good for primary cells? Does it yeast contamination? How to get rid of them without killing the cells? How to get rid of spores?

Hello! Our lab is working with primary cells which we are isolating from the colorectal tissue. During the isolation, we are washing the tissue with pen-strep and then also add some pen-strep in...

07 February 2021 8,258 3 View

Which is the best software for the simulation of tribological properties of materials?

I want to simulate the tribological properties of solids, liquids, and fluids, please suggest good software.

02 February 2021 3,947 3 View

Does anybody work with CoolLED in fluorescence microscopy?

I have trouble with exciting wavelengths higher than 460 nm while e.g. ex with 405 nm works. Filtercubes are correc, as I used them with my old xenon lamp before... I work on axiovert200M and with...

25 January 2021 5,201 2 View

How to prepare a liquid sample for FTIR spectrum?

I have a ethanol-water 50% extract of mango peel. I purified mangiferin from the extract with filtration and precipitation. so i obtained a low concentrated extract which i want to se the IR...

24 January 2021 8,880 7 View

Why high voltage and current (and some gases) Cause Spray-mode metal transfer in Gas-Metal Arc Welding (GMAW)?

This transition phenomena of Globular to spray mode as voltage , and especially current is raised, is very commonly known to Manual Metal Arc Welding (MMAW) and Gas-Metal Arc Welding (GMAW)...

20 January 2021 7,984 2 View

Can you have pressure inlet and velocity inlet at the same time?

I was reading a reference (Reitz-A Photographic Study of Flash Boiling Atomization). In his experiment, his boundary condition is delta p and constant velocity. The liquid injection pressure was...

18 January 2021 842 9 View