We are trying to coat the substrate of PMMA by using HA in order to collect high concentration of platelet in Platelet-Rich Plasma and preventing the platelet adhesion on the surface of the substrate.

We need to overcome the hydrophobic interaction of the substrate (PMMA) by modifying the surface with the proper solution in which this solution can not affect or contaminate the platelet when we collect them because we are dealing with human blood.

We used NaOH but the HA still not coated well or spread on whole the substrate surface.

So, if there is anyone who dealt with such a thing like this can please help us.