One way is to subclone the GPCR of interest and perform C terminus truncation using standard PCR-based mutagenesis (or if you're lucky there may be a restriction enzyme site conveniently located near the truncation site). You can then heterologously express back the mutant GPCR using transfection, transduction or electroporation, and compare function between wt and mutant(s).

Thanks for the reply but we are looking to cleave in in vitro or in vivo sytem......to make it more clear we want to cleave it in cells transfected with receptor and then to check its translocation...............and also in mouse tissues...can we do that