Raise Ahmad

5 Questions 6 Answers 0 Followers

Questions related from Raise Ahmad

Very small peptide detection in solution?

I am looking for an assay (rapid and convenient) where I can quantify/semiquantitative amount of very small peptides (dipeptides to tetrapeptides) in solution. I have tried different commercial...

10 November 2020 6,334 6 View

Does C18 desalting column can be used to remove amino acids and ions?

Hi there, I want to remove any ions (mostly Ca2+, amino acids) from my soluble protein extract preparation. I came across with idea of using C18 column which only binds to peptides and rest will...

26 July 2020 9,990 3 View

How to chelate free calcium in my sample?

I want to study the effect of free calcium ion in pharmacological cell based assay. I have around 15mM calcium in my samples. Can anybody suggest how can I chelate with chelating agent (EDTA, EGTA...

28 June 2020 3,948 3 View

Can someone help me with a mice cardiomyocytes culture protocol?

I am trying to establish cardiac cell or cardiomyocytes isolation and culture from a mouse heart. I need a convenient protocol, if somebody can help me out. As far as I know the heart has to be...

12 September 2013 7,059 1 View

ROS production in brain.

What are the most convenient methods to check Oxidative stress in brain tissue?

09 April 2013 9,570 4 View