Raise Ahmad

7 Questions 8 Answers 0 Followers

Questions related from Raise Ahmad

In Vivo tracking of mitochondria?

Dear all, We are interested in exploring in vivo mitochondrial functions in disease state. For that we want to track live mitochondria physiological system or in particular tissue or lets say say...

23 June 2021 9,535 3 View

Very small peptide detection in solution?

I am looking for an assay (rapid and convenient) where I can quantify/semiquantitative amount of very small peptides (dipeptides to tetrapeptides) in solution. I have tried different commercial...

10 November 2020 6,347 6 View

Does C18 desalting column can be used to remove amino acids and ions?

Hi there, I want to remove any ions (mostly Ca2+, amino acids) from my soluble protein extract preparation. I came across with idea of using C18 column which only binds to peptides and rest will...

26 July 2020 10,001 3 View

How to chelate free calcium in my sample?

I want to study the effect of free calcium ion in pharmacological cell based assay. I have around 15mM calcium in my samples. Can anybody suggest how can I chelate with chelating agent (EDTA, EGTA...

28 June 2020 3,965 3 View

Does anybody have an idea how to analyze tumors and metastasis in MMTV mouse models?

We are trying to see change in MMTV mouse with our gene knockout. Can anybody suggest how to proceed with it. I mean what would be the strategy to observe the changes in terms of number/size of...

05 April 2015 4,142 6 View

Can someone help me with a mice cardiomyocytes culture protocol?

I am trying to establish cardiac cell or cardiomyocytes isolation and culture from a mouse heart. I need a convenient protocol, if somebody can help me out. As far as I know the heart has to be...

12 September 2013 7,068 1 View

ROS production in brain.

What are the most convenient methods to check Oxidative stress in brain tissue?

09 April 2013 9,578 4 View