I have one polar product with an impurity, which has the similar structure and polarity. I used the Hexane:Ethyl acetate:Methanol in 6:3:1with silical gel. And the difference of Rf is around 0.08, which may be too small for running regular silica gel column for separation. And then I was thinking to use C18 column for separation these two polar compunds, any suggestion for solvent choice? Or the procedure to try the solvent?
Thank you.