How to choose reference genes when doing qPCR?? I found even commonly used reference gene GAPDH sometimes have ups and downs in different cell lines? For example, right now I am comparing different reference genes in different cell lines and found GAPDH did not work very well.
Sang Ho Lee
Hello, Fei.
That's right. Specially when you have treatment groups in experiments.
There are already some discussions about the issue. Have a look. https://www.researchgate.net/post/Would_alcohol_administration_affect_GAPDH_expression_at_the_protein_level
All the best.
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