How to deal with the recudtion of amplification efficiency of indexed primers?

Hi all, I am now working with ITS2 sequencing library preparation. I had observed that after introducing adapters, linkers and barcodes to a primer, the indexed primers have a much lower...

27 January 2021 5,045 3 View

Asalam o alikum, Can anyone please help me in interpretation of XRD data?

Actually, I want to present the in table form (dcal , dexp, 2 thita, hkl) but don't know how to find these values from data.

28 December 2020 6,769 4 View

Is it need to test Cronbach's Alpha for extracted features from data?

As Cronbach's Alpha is usually used for test the reliability of measurements given by questionnaires, however, I wonder that whether I use this test for features extracted from behavioral data. I...

26 December 2020 9,602 2 View

Are there any DNA intercalators that do not have fluorescence?

I understand several normal DNA intercalators, such as ethidium bromide, acridine, SYBR et al. would emit fluorescence. Is there any kind of DNA intercalator that do not have fluorescence?

25 December 2020 688 3 View

Doping of r-GO?

if the percentage of doping Au into rGO is too small 1~10%, is it not enough to prevent restacking of r-GO in xrd results, rGO peak appeared at 22.2 and also the peaks of gold nanoparticles so...

21 December 2020 6,091 3 View

XRD peaks for gold nanoparticles ?

is the concentration of gold salt that were added will affect on the peaks appeared. i added a very little amount of gold salt to dope graphene oxide and not all the peaks that proved the...

17 December 2020 9,275 3 View

If someone has the pdf file of below mentioned reseach paper?

14 December 2020 5,935 3 View

How many times should I inject the drug before I take samples for RNA-Sequencing?

I'm going to perform RNA-sequencing of the zebrafish brain with three different treatments, which are naive fish, alcohol withdrawal fish injected with Saline, alcohol withdrawal fish injected...

08 December 2020 4,643 2 View

How do I transform the "Transcripts" into the "Gene symbol"?

I downloaded the expression matrix data "GSE143416_Brain_data_TPM.txt.gz" of GSE143416 from Gene expression omnibus ( https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi). However, the first column of...

30 November 2020 1,304 3 View

I saw sparkles in my samples?

i am trying to dope boron in graphene oxide , and after i take out my samples from the oven i put them in the microwave for 40 sec , and i saw sparkles . and fume also i couldn't understand why

16 November 2020 6,852 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How the wettability of rock sample can be changed by ageing in pure hydrocarbon oils only?

I want to do wettability analysis of hydrocarbon oil (octane)/rock/surfactant solution system. but to do the analysis first I have to make rock surface oil-wet. Since hydrocarbon oil does not have...

27 February 2021 5,383 3 View

What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?

24 February 2021 8,219 2 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

How can Gamma Ray API units be converted to CPS (counts per second)?

Oil and gas companies run gamma ray tool and take readings in API units while Gamma ray is also noted in cps, so how these units can be converted into each other?

24 February 2021 7,545 2 View

Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software?

Hi every one Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software? Biogas fuel is not available in the options thanks

23 February 2021 6,518 3 View

How to remove compound ( Europium complex ) from paraffin oil?

How can I remove my compound from paraffin oil? Mistakenly my compound fell in an oil. Compound = Europium complex

22 February 2021 5,667 2 View

When I mix oil and NaOH solution, Why solid layer in the surface is formed?

During my trial for lubricant oil re-refinery process, I use NaOH as coagulant agent. After overnight of gravity settling, a solid layer in the surface was form. what product is that? since the...

22 February 2021 2,116 4 View

The type of filter paper used to filter an epoxidized oil (the mixture is an epoxidized oil with magnesium sulfate) ?

I have a mixture of epoxidized oil and magnesium sulfate wich type of filtre paper should i use

21 February 2021 3,944 3 View