Our department have Guava easyCyteTM8 flow cytometer which has a 488 and 640 laser and various shutter as described in link, https://www.aub.edu.lb/fm/medicalresearch/Pages/Guava.aspx , we routinely use FITC and propidium iodide for staining.

Recently, we need to stain for more target, in this context we seek to search available dye, but other from these two also in regard to the laser, is hard to find a dye that's slightly overlap with.

Does anyone have suggestion regarding fluorescent dye?

Also, how much the difference in nm is unable to excite or result to undetectable emission wavelength? e.g. 488nm to a 495/519 dye.

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