Hi,
I am injecting AAV2-Retro virus (AAV2-retro-EF1a-DIO-FLPo-WPRE-hGHpA from Addgene) that carries cre-dependent flp recombinase, in the mouse dorsal hippocampus and I need to verify that the spread is limited to the hippocampus. When the virus infects the cells in the hippocampus, there will not be any fluorescence expression because the viral construct has no fluorophore. I am of course exploring alternatives to this virus so there will be fluorophore expression. However, I was wondering if there is any other easy way to check the viral spread. Has anyone tried any antibodies against AAV2 proteins?
Thanks
Mani
Yes u can use AAV2 antibodies via immunoflourescence or western blots to look at AAV2 expressed proteins.
I used this before:
https://www.biocompare.com/9776-Antibodies/4659071-AAV-VP1-VP2-VP3-antibody/?pda=9776|4659071_0_1||2|AAV&dfp=true
And it works well. you can try too.
Hello,
You could also test the tissue using in situ hybridation (FISH) targeting any unique sequence present in your viral construct.
Hope this hepls
