How to check MIC of different anti-fungal drugs against Mucormycosis ?

Rahul Badgha @Rahul-Badgha

05 July 2021 1 2K Report

Can anyone help me with protocol ?

Thank you in advance.

Gillian Dumsile Mahumane

Rahul Badgha check:

Clinical and Laboratory Standards Institute. Reference Method for Broth Dilution Antifungal Susceptibility Testing of Filamentous Fungi; Approved Standard, 2nd ed.; CLSI document M38-A2; Clinical and Laboratory Standards Institute: Wayne, PA, USA, 2008
Also cited by doi.org/10.3390/jof7040271

Badges
Science topic

More Rahul Badgha's questions See All

What are the roles of both Monetry and Fiscal Policy coordination in optimising the Macroeconomic outcomes in the economy ?

These days policy coordination is becoming necesssary on different front. In India also it is the same case. Monetary and Fiscal policy interface is important in order to address the...

30 July 2024 2,104 6 View

Can we fit a gaussian Fit on a LIBS Peak ?

Though much literature suggests fitting a Voigt profile on a LIBS peak, is it also possible to fit a Gaussian profile on the LIBS peak? I have 204 spectra files, and I am building a pipeline for...

23 July 2024 6,127 1 View

Can the MDFT mental health care model help reduce drug addiction?

This question might come up in my research on "Frustration, Fantasy, and Drug Addiction," where I'm also trying to figure out how to overcome mental health issues without turning to drugs.

30 June 2024 5,936 0 View

What is the effect of redshift on the evolution of an AGN?

AGN (Active Galactic Nuclei) are high energy galaxies powered by supermassive blackholes. The evolution of a galaxy is characterized by parameters such as luminosity and redshift. The redshift is...

25 June 2024 3,782 2 View

Which smoothening model is acceptable for processing of circular dichroism spectra?

For smoothening of noisy CD spectra, several fitting models are available in Jasco spectra manager software, like Savitzky Golay, binomial, means movement and adaptive smoothening. Each one asks...

16 June 2024 3,997 1 View

I am carrying out MTT Assay using HeLa cells but the absorbance values are really less, can anyone please help?

I have seeded HeLa cells in a calculation of 10000 cells per well in a 96 well plate. Then I went for treatment in the next day. Then at last, I added 7ul of 5mg/ml solution of MTT reagent into...

12 June 2024 1,460 3 View

Can we use LIBS Peak - Ti 441.492 for the Univarate Analysis ?

I have 17 different steel samples, in which i identified a peak of Ti 441.492. But , if you look closely at photo, while some some peak are at 441.492 , some are at the slight offset. eg 441.490...

10 June 2024 8,934 2 View

Challenges in Univariate Analysis for Stainless Steel Samples using Laser Induced Breakdown Spectroscopy?

Respected Members , I have a 17 different steel sample references , with varying concentration for Minor Elements being Mo , Ni , Mn ,Si , Cr etc , i am trying to build a calibration curve with...

10 June 2024 993 0 View

Is radio emission thermally or non thermally dominated?

Thermal emission is temperature dependent. Non-thermal emission is not temperature dependent such as synchrotron emission.

29 May 2024 5,155 2 View

Normalization in LIBS Spectra ?

Hello Everyone ! I have a samples a satinless steel , I performed the LIBS Measurement with hLIBS (SciAps Z-300). Total of 12 measurements on different locations were done on the same sample...

09 May 2024 7,959 6 View

Is changing the medium during the experiment overestimate the results?

I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...

07 August 2024 2,283 2 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

Is it necessary to attach fluorophore at 3' end in MIC RT PCR machine for Dual hybridization Probe designed especially for genotyping assay?

I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...

28 July 2024 5,658 0 View

How to calculate FIC index?

Can someone explain to me how was the MIC of MO in combination and MIC of antibiotic in combination was calculated? as shown in the attached file below.

26 July 2024 2,702 1 View

How to maintain the drug concentration in cell culture study for long term?

I am working in the filed of long acting drugs with Nano formulation. I want to study the efficacy of my formulation in 2D cell cultures while this required media change on every two days. In such...

24 July 2024 8,696 0 View

How can I determine if a drug binds to a ribosome using a Cryo-EM map?

Hello, I am searching for the binding position of a drug in a ribosome, based on previous work indicating it binds there. The resolution of the ribosome-drug Cryo-EM map is around 2.5 Å. I've been...

19 July 2024 2,155 0 View

How to calculate percentage cumulative drug release?

How to calculate percentage cumulative drug release when, Total volume of dissolution media = 250ml Added formulation was equivalent to 10mg of drug Sample withdrawn = 1ml each time Sink condition...

15 July 2024 4,192 1 View

MTT assay - 6 well plate?

Hi, In the 6 well plate, when I am seeding the cells. Do I add 1ml of complete media to one of the wells for the control at this stage or do I wait unit I am treating the plates to do that? Also...

13 July 2024 9,783 1 View

How to convince decision makers to adjust the legislation to allow opening of drug consumption rooms?

In my country, there is a lot of public outcry in relation to the presence of drug injection and related behaviors in public. Hence at least the bigger cities would probably benefit from the...

08 July 2024 8,903 1 View