How to carry out protein crosslinking with Copper (II) phenanthroline?

More Kasturi Pal's questions See All

How to determine tannin content in fodder pellets?

22 July 2024 8,162 3 View

How to avoid background signal in screen printed electrode?

Hi, I am doing aptamer based electrochemical biosensor. First, I cleaned screen printed gold electrode using 0.5M H2SO4. Then, immobilized aptamer followed by blocking using 2mM MCH. I use...

06 July 2024 9,852 1 View

Want to authenticate aquatic plant species Duckweed? Two University botanist are unable to confirm, any confirmed place where it can be done in India?

06 June 2024 5,845 0 View

How do we quantify the flow behavior in a drying drop?

Is it possible to quantify the flow without adding any polymeric beads? I'm interested to know whether there's dominant capillary or Marangoni flow. Aby suggestions would really be helpful.

05 May 2024 2,233 5 View

Today I found a very strange thing in my research gate account..it showing Citations -0..However, I saw more than 150 Citations last week. How to fix?

Today I found a very strange thing in my research gate account..it showing Citations -0..However, I saw more than 150 Citations last week..Dose anyone know how to fix it ??

19 April 2024 1,111 2 View

Alphascreen versus TR-FRET?

I want to quantify low cAMP levels generated of a constitutively active GPCR also by different mutants of this receptor. I have tried the cAMP ELISA kit from Cayman chemicals with very...

14 April 2024 7,319 0 View

Has AI taking a big leap in Science and Engineering?

AI is coming fast, and we have to adapt.

29 March 2024 2,315 1 View

Bone material: What this minimum of maximum strain of Human cortical Bone before fracture?

What this minimum of maximum strain of Human cortical Bone before fracture. I saw lot of papers showing different values.

27 March 2024 4,255 1 View

Autodock Vina Grid Spacing is 0.375 A or 1.0 A?

Hello everyone, I am conducting molecular docking using Autodock Vina. The receptor (protein) is huge therefore the grid spacing 0.375 A is not enough (Autodock Tools). So, I also increased the...

22 March 2024 8,368 1 View

Does the presence of degenerate nucleotides in consensus sequences affect the results of population genetic analysis?

I want to run STRUCTURE on a nuclear gene of an insect species. The sequences that I have received are showing the presence of degenerate nucleotides in the consensus sequences. Do I need to edit...

29 February 2024 648 1 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,494 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,726 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,703 7 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

How to restart MD without using checkpint file in GROMACS?

Dear All Gromacs User I would like to restart MD without using checkpint .cpt files? I heard there is a way for acheiveing this. Any input and sugegstions are most welcome. Thanking You Budheswar

29 July 2024 3,083 8 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

AutoGrid and Autodock 4.2.6 window opens for 1 second then closes and there is no output files?

Good day, I am a student trying to work on Autodock for a project regarding Ligand-DNA interaction so i am quite new to molecular docking. i have followed tutorials and did all the steps...

28 July 2024 2,136 4 View

Adam B Shapiro

Here is an example of a paper in which this method was used.

http://www.jbc.org/content/279/9/7692.full

Kasturi Pal

Thank you Adam. I wonder why after the initial 24 hrs of transfection, the cells need to be incubated at 27 degrees for the next 24 hours?

I don't know the reason. I suggest you contact the authors as ask them.