I need to know how to set zero pressure before inserting the catheter to the carotid artery, So that I could get the accurate BP changes from artery.
please check it
Article Catheterization of the Carotid Artery and Jugular Vein to Pe...
Dear Sir/Madam,
It shows the catheterization of artery which will be done by experienced veterinarian. We need to calibrate the pressure transducer for the proper readings of Invasive blood pressure measurement.
Calibration is one of the important steps in the experiment, and it will reflect in the experimental results. Calibration is performed using a known pressure levels with the help of a sphygmomanometer. The pressure cuff of the sphygmomanometer is removed and connected to the pressure transducer of the student physiograph/data acquisition system. Then, the pressure transducer is checked by inflating to a known pressure level. The conversion factor for calculating BP is determined. In the case of the data acquisition system, the calibration between the voltage (millivolts) and the pressure is performed previously, and the results are automatically calculated based on the calibration value.
Parasuraman S, Raveendran R. Measurement of invasive blood pressure in rats. Journal of Pharmacology & Pharmacotherapeutics. 2012;3(2):172-177. doi:10.4103/0976-500X.95521.
Thank you sir, we read that paper already. But the thing is when it is calibrated, Where should I keep the transducer? because altitude changes has effect in pressure and also distance from catheter and Should I maintain positive pressure like 5mmHg, or Baseline 0 mmHg, before starting the procedure?
The best is with a mercury column connected with a sphygmomanometer. Very precise and you may use a clamp to adjust the pressure. Once calibrated put the transducer wherever you want. In its box if you have to move it. What differences in altitude are you speaking about ?
Here altitude means height of pressure transducer from the catheter. It must be of same height. Am I rgt?
Yes but if the difference is only a few centimeters it is still ok. Don't worry too much.
Even a change in position of about 1 mm causes change in pressure sir. But anyhow, Calibration is successful and Thank you all for your efforts to answer my question. Please respond my further queries too...
No, it should not be detectable, as 1mm of mercury is equivalent to 13,6 mm of water.If you see any variation in pressure in your conditions, it s due to something else.
Sir, I used Pressure Gauge for the calibration of pressure transducer attached to the Data Acquisition system. I told that if the catheter is slightly moved up or down, there is change in pressure.
TSD104A is the pressure transducer and it is connected to Bourdon gauge using tubing.
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