I'm looking for N-substition reactions of carbazole, does anyone have any tips for these in order to get efficent yield and simple synthesis protocol?

I want to synthesize N-phenyl Carbazole

31 December 2017 6,880 6 View

How to remove chloramine T?

06 July 2016 881 3 View

Solvent-accissable void volume?

How can I calculate void space volume using palton?

31 December 2015 7,590 0 View

On the basis of the 1H NMR spectrum, how to estimate that he quantity of absorbed solvent?

09 October 2015 5,069 0 View

What are other causes of red shift in fluorescence spectra of chromophore apart from pi-pi stacking?

My sample contains anthracene derivative with a solvent means solvate, I have done SXRD there is no such pi--pi stacking but it showed red shift don't know how to explain. if someone have...

05 June 2015 502 7 View

What is structure factor or Rietveld data file in CIF format?

I want to submit paper to one of willey journal, I successfully upload Cif then the system asked me to upload structure factor or Rietveld data file in CIF format. I don't know that. Need help

05 June 2015 3,071 0 View

How to dissolve?

I crystallize my compound from methanol one year before now I want to dissolve it in the same solvent it didn't work out . Any suggestion please on how to dissolve it again

04 May 2015 5,017 0 View

Can someone suggest a book about basics of crystallography?

Can someone suggest me or share here book about basics of crystallography. I don't have major in crystallography.

03 April 2015 4,470 10 View

Can anyone help me how to describe the SEM image?

I have done SEM analysis for my crystal, don't know how to describe it and which image should I put in paper. Thanks

03 April 2015 4,134 5 View

Why the fluorophore has a larger dipole moment in the excited state?

Can anyone tell me

01 February 2015 426 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Can polymer concentrations be calculated based on the number average molecular weight?

Hello, I spin-cast two different polymers onto wafers and measure their height as a function of initial concentration. For polymer A, I have the weight-average molecular weight (Mw) as well as...

28 February 2021 7,882 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View