How to calculate total soluble protein content by the method of Lowry et.al?

24 March 2021 1 6K Report

I have different OD values and different tissue weight.. and when calculating it the lower tissue weight content the greater value thn the higher tissue weight !! I couldn't get the proper results..can anyone help me to find out results!!??

Thomas Nicol

What method have you used to measure your absorbance?

Also, note "...the total concentration of protein in the sample can be deduced from the concentration of tryptophan and tyrosine residues that reduce the Folin–Ciocalteu reagent."

The concentration of the residues reduce the reagent's concentration. If you calculate in terms of the total amount instead of the amount reduced you can run into problems.

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