How to calculate total protein in dry fish if we have only the data of total amino acids present in dry fish. Is there any method to calculate?
The sum of all indidual amino acids is the amount of protein. But where they all individually determined?
The amount of ptotein equals the crude protein (nitrogenous compounds, including amino acids) plus non-protein nitrogenous compounds as urea ..!
Marwa Hussein I totally disagree with you that non-protein nitrogen is part of the total protein. The name itself already says it clearly: non-protein!
This link might also help: https://en.wikipedia.org/wiki/Protein
Mr. C. A (Kees)
Sir amino acids quantification was done solely by HPLC. Whether summation of those amino acids quantitatively will provide protein value?
If all amino acids were determined, then the sum of all of them is a very good approximation of the protein content.
Be aware that generally the protein content is calculated by determining the N content and then multiplying by a certain factor based on the average amino acid composition of proteins. So this might also deviate from the "real" value.
Next to that all analyses have their margin of uncertainty, so do not worry too much.
Is it possible to determine all amino acids? Is there any method from which we can get all amino acids?
Actually, we generally analyze all components which have nitrogen
by Kjeldahl methods and we present as crude protein content of the diet. However, to determine the amino acid profile chromatographic methods and different analysis methods for urea content can be used.
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