In several reports it is mentioned that traditional MS media contains 60 mM Nitrogen, if I wants to make MS media with 50, 40 ,30 and 20 mM total nitrogen, How can I make it, if someone help me in calculation please?
Dear Mr. Mohamed, did you mean amount of KNO3 and NH4NO3 in MS medium?
If you mean this amount. It will be 40 mM not 60 mM.
If you need explain. Please contact me. Thank you and good luck.
Please see you attachment files. Thank you and good luck.
Thank you so much sir, for your time and efforts it will help me a lot
Dear Muhammad Irshad,
Look the link, may be useful.
http://www.academicjournals.org/article/article1380729549_Shirdel%20et%20al.pdf
Regards, Shafagat
I think so
Hello Muhammad Irshad,
please check the link
https://plantmethods.biomedcentral.com/articles/10.1186/s13007-019-0473-1
Article Role of reduced nitrogen for induction of embryogenic callus...
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