Dear Mr. Mohamed, did you mean amount of KNO3 and NH4NO3 in MS medium?

If you mean this amount. It will be 40 mM not 60 mM. 

If you need explain. Please contact me. Thank you and good luck. 

Please see you attachment files. Thank you and good luck. 

Thank you so much sir, for your time and efforts it will help me a lot

Dear Muhammad Irshad,

Look the link, may be useful.

http://www.academicjournals.org/article/article1380729549_Shirdel%20et%20al.pdf

Regards, Shafagat

I think so

Hello  Muhammad Irshad,

please check the link

https://plantmethods.biomedcentral.com/articles/10.1186/s13007-019-0473-1

Article Role of reduced nitrogen for induction of embryogenic callus...