8 Questions 4 Answers 0 Followers
Questions related from Maria Elisabetta Michelangeli
Hi, I have been analyzing tot glutathione peroxidase (GPx) activity in mussel cells using cumene hydroperoxide (microplates) and I am a bit unsure how to read/calculate the results. I run a...
04 April 2024 9,290 0 View
Hi, the fluorescent probe BODIPY® 581/591 once oxidized shifts from red to green fluorescence and it localizes in the lipid bilayer and does not spontaneously leave it C11-BODIPY581/591, an...
02 August 2022 136 0 View
Hi, Im using these two probes to respectly detect neutral lipids and lysosome presence in bivalves haemocytes by using flow cytometry. I have some doubts regarding the depletion/increase of the...
14 February 2022 5,360 2 View
Hi, I used for the first time the Annexin V-FITC Apoptosis Detection Kit (BioVision) but I am a bit unsure of the results. I investigated the apoptosis on mussel M. edulis haemocytes, by using...
03 December 2021 4,435 3 View
Hi, I stained bivalves haemocytes with PI (final concentration 10ug/mL) , SYBR Green (final concentration 1x and 10x) and PI+SYBR Green. The concentration I used are the ones commonly used in...
22 March 2021 8,849 4 View
Hi, In literature I noticed that who stains haemocytes with FDA for 30 min then stop the reaction in ice while this does not apply for 15 min incubation time (or maybe authors did not write this...
09 February 2021 3,596 3 View
I am trying to optimize a flow cytometry method to characterize mytilus spp. haemocytes. In literature I found that someone, to avoid clotting, use anti-clotting solutions or dilute and fix the...
06 November 2020 2,633 4 View
Hi, I'm using a BD Accuri C6 flow cytometer and I'm trying to analyze the hemocyte population of the mussels, I tried the threshold at 2500, 10000 and 50000. but I can't get clear results, it...
06 May 2019 1,038 4 View
