About COMSOL , HOW TO COUPLE EQUATION?

26 January 2021 4,690 3 View

How long does Hela cancer cells take to reach 80% confluency after thawing from liquid nitrogen?

Three days ago, i thawed Hela cancer cells from liquid nitrogen but until now they are still suspended in the media and very very low number of cells became attached to the tissue culture...

06 January 2021 6,511 4 View

Any recommendations for a good CRISPR/Cas9 commercial system for functional genomics of fungi/yeast?

I look forward to implying a good CRISPR/Cas9 system with a selective maker for functional genomics of important genes from environmental fungi and yeast.

22 December 2020 1,432 2 View

What is the appropriate media for WI-38 cell line?

Iam going to culture WI-38 ( a normal lung cell line), it is the first time for me to deal with this cell line so i want to know the most suitable media for this cell line? In addition to, if i...

18 December 2020 6,542 3 View

Can i use an expired fetal bovine serum?

I have fetal bovine serum expired from many years ago but it kept at -200C during all this period and did not expose to cycles of freezing and thawing. So, can i use it for cell culturing? In...

18 November 2020 1,237 7 View

How can i solve the problem appeared during autogrid run using cygwin?

This is the first time for me to use the autodock software. I used autodock tools 1.5.6 I prepared pdbqt file for my ligand and my target and also file for grid parameters and docking parameters,...

02 September 2020 5,914 2 View

How do you think our Architectural spaces would change after COVID 19?

How do you think our Architectural spaces would change after COVID 19?

10 May 2020 3,172 27 View

How do i avoid glass pipette being blocked by brain matter during virus injection?

Hello everyone, I am injecting lentivirus into the hypothalamic region of the mouse brain. I am going in DV -6.05 mm and, more often than not, I have brain matter clearly clogged at the tip of...

24 January 2020 2,522 3 View

When do you increase the number of samples?

Using the short-time Fourier transform (spectrogram) for different sample sizes

19 October 2019 3,799 2 View

How to measure the level of IGF-1 in PC3 cells?

Dear All, Does anyone share the experience about the measurement of IGF-1 in cells? I tried several times for measuring the level of IGF in cells by using the cell lysate or medium. However, I...

08 September 2018 7,348 3 View

How the wettability of rock sample can be changed by ageing in pure hydrocarbon oils only?

I want to do wettability analysis of hydrocarbon oil (octane)/rock/surfactant solution system. but to do the analysis first I have to make rock surface oil-wet. Since hydrocarbon oil does not have...

27 February 2021 5,383 3 View

What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?

24 February 2021 8,219 2 View

How can Gamma Ray API units be converted to CPS (counts per second)?

Oil and gas companies run gamma ray tool and take readings in API units while Gamma ray is also noted in cps, so how these units can be converted into each other?

24 February 2021 7,545 2 View

How to remove compound ( Europium complex ) from paraffin oil?

How can I remove my compound from paraffin oil? Mistakenly my compound fell in an oil. Compound = Europium complex

22 February 2021 5,667 2 View

When I mix oil and NaOH solution, Why solid layer in the surface is formed?

During my trial for lubricant oil re-refinery process, I use NaOH as coagulant agent. After overnight of gravity settling, a solid layer in the surface was form. what product is that? since the...

22 February 2021 2,116 4 View

The type of filter paper used to filter an epoxidized oil (the mixture is an epoxidized oil with magnesium sulfate) ?

I have a mixture of epoxidized oil and magnesium sulfate wich type of filtre paper should i use

21 February 2021 3,944 3 View

What reaction will occur when adding hexamine into ammonium nitrate in an acidic medium?

Hello, I am investigating properties of water in oil emulsion explosives. And adding hexamine (hexamethylenetetramine) into one of the phases that are used to make the emulsion should increase the...

21 February 2021 8,680 1 View

How can we study the adsorption efficiency of microgel at the oil-water interface?

I am studing the mircogel at oil-water interface. I want to know the adsorb amount of microgel at the oil water interface. How we can determine this amount?. I will highly appreciate your...

19 February 2021 2,787 1 View

Does anyone know, can i do cryosectioning of whole aorta after oil red O staining and use the cryosection parts for further analysis?

I would like to quantify the lesions on aorta. I want to take image of atherosclerotic plaques formation in aorta by using O red Oil Solution. After the staining, what will be the storage...

16 February 2021 5,876 2 View

Can any one explain why the ethyl ester Biodiesel (FAEE) has higher than that of the metyl ester FAME- both of them are derived from the same oil?

Biodiesels derived from waste vegetable oil transesterification with Ethanol (FAEE) show higher peroxide values than those derived by transesterification with methanol (FAME) prepared under the...

13 February 2021 193 6 View